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Western Blot analysis of 293 cells using RACK7 Polyclonal Antibody
Western Blot analysis of 293 cells using RACK7 Polyclonal Antibody
Western Blot analysis of 293 cells using RACK7 Polyclonal Antibody

ZMYND8 Antibody

CSB-PA003909
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetZMYND8
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Overview

  • Supplier
    Cusabio
  • Product Name
    ZMYND8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23613
  • Target name
    ZMYND8
  • Target description
    zinc finger MYND-type containing 8
  • Target synonyms
    PRKCBP1, PRO2893, RACK7, MYND-type zinc finger-containing chromatin reader ZMYND8, CTCL tumor antigen se14-3, RACK like clone 7, cutaneous T-cell lymphoma-associated antigen se14-3, predicted protein of HQ2893, protein kinase C-binding protein 1, transcription coregulator ZMYND8, zinc finger MYND domain-containing protein 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULU4
  • Protein Name
    MYND-type zinc finger-containing chromatin reader ZMYND8
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Hela cell lysates; Lane 2: ZMYND8 knockout (KO) HeLa cell lysates probed with ZMYND8 Polyclonal Antibody, Unconjugated (bs-55227R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYND8 antigen affinity purified polyclonal antibody (Catalog # A06830-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZMYND8 at approximately 150 kDa. The expected band size for ZMYND8 is at 132 kDa.
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