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KPNB1 Antibody

CSB-PA012490GA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetKPNB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KPNB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3837
  • Target name
    KPNB1
  • Target description
    karyopherin subunit beta 1
  • Target synonyms
    IMB1, IPO1, IPOB, Impnb, NTF97, importin subunit beta-1, PTAC97, importin 1, importin 90, importin beta-1 subunit, karyopherin (importin) beta 1, nuclear factor p97, pore targeting complex 97 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14974
  • Protein Name
    Importin subunit beta-1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KPNB1 using anti-KPNB1 antibody (A01851-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNB1 antigen affinity purified polyclonal antibody (Catalog # A01851-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KPNB1 at approximately 97 kDa. The expected band size for KPNB1 is at 97 kDa.
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ReactivityHuman, Mouse, Rat
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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA244093(KPNB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Importin beta 1 antibody (GTX133733) diluted at 1:1000 and competitor's antibody (sc-11367) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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