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KRIT1 Antibody (YA3147, Rabbit)

HY-P83402
MedChem Express
TargetKRIT1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KRIT1 Antibody (YA3147, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID889
  • Target name
    KRIT1
  • Target description
    KRIT1 ankyrin repeat containing
  • Target synonyms
    CAM, CCM1, krev interaction trapped protein 1, ankyrin repeat-containing protein Krit1, cerebral cavernous malformations 1 protein, krev interaction trapped 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    KRIT1 Antibody (YA3147) is a non-conjugated IgG antibody, targeting KRIT1, with a predicted molecular weight of 84 kDa (observed band size: 84 kDa). KRIT1 Antibody (YA3147) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KRIT1 using anti-KRIT1 antibody (PB10074). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: NIH3T3 whole cell lysates, Lane 3: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRIT1 antigen affinity purified polyclonal antibody (Catalog # PB10074) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRIT1 at approximately 84 kDa. The expected band size for KRIT1 is at 84 kDa.
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