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Western blot analysis of extracts of various cell lines using KRT12 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using KRT12 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using KRT12 Polyclonal Antibody at dilution of 1:1000.

KRT12 Polyclonal Antibody

E-AB-65092
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    KRT12 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
  • UNSPSC
    12352203

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Immunofluorescence staining of Hela cells with CSB-PA859510LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of KRT12 using anti-KRT12 antibody (M05227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT12 antigen affinity purified monoclonal antibody (Catalog # M05227) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT12 at approximately 54 kDa. The expected band size for KRT12 is at 54 kDa.
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