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ProductsBack/KTEL1 Polyclonal Antibody
Lane 1: Mouse Cerebrum lysates probed with KTELC1/KTEL1 Polyclonal Antibody, Unconjugated (bs-16861R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates probed with KTELC1/KTEL1 Polyclonal Antibody, Unconjugated (bs-16861R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates probed with KTELC1/KTEL1 Polyclonal Antibody, Unconjugated (bs-16861R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

KTEL1 Polyclonal Antibody

BS-16861R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPOGLUT1
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Overview

  • Supplier
    Bioss
  • Product Name
    KTELC1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID56983
  • Target name
    POGLUT1
  • Target description
    protein O-glucosyltransferase 1
  • Target synonyms
    C3orf9, CLP46, KDELCL1, KTELC1, LGMD2Z, LGMDR21, MDS010, MDSRP, Rumi, hCLP46, protein O-glucosyltransferase 1, 9630046K23Rik, CAP10-like 46 kDa protein, KDELC family like 1, KTEL (Lys-Tyr-Glu-Leu) containing 1, KTEL motif-containing protein 1, O-glucosyltransferase Rumi homolog, hRumi, myelodysplastic syndromes relative protein, protein O-xylosyltransferase POGLUT1, x 010 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBL1
  • Protein Name
    Protein O-glucosyltransferase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of POGLUT1 using anti-POGLUT1 antibody (A06826-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGLUT1 antigen affinity purified polyclonal antibody (Catalog # A06826-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGLUT1 at approximately 55 kDa. The expected band size for POGLUT1 is at 55 kDa.
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