Ku70 antibody [N3H10]

GeneTex

Ku70 antibody [N3H10]

9

Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2microg/mg of protein lysate (native and denatured). Western blotting: use at a concentration of 0.25 - 0.5microg/ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2microg/ml for 30 minute at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min prior to immunostaining. By Western blot, this antibody detects a band of 70 kDa, which corresponds to the predicted molecular weight of Ku 70.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

N3H10

0.2 mg/ml

Unconjugated

XRCC6

X-ray repair cross complementing 6

5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; KU70; lupus Ku autoantigen protein p70; ML8; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6

Mouse

IgG2b

X-ray repair cross-complementing protein 6

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Hamster, Human, Monkey, Mouse, Xenopus

2°C to 8°C

12352203