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Western Blot analysis of KB cells using CD305 Polyclonal Antibody
Western Blot analysis of KB cells using CD305 Polyclonal Antibody
Western Blot analysis of KB cells using CD305 Polyclonal Antibody

LAIR1 Antibody

CSB-PA006294
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetLAIR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LAIR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3903
  • Target name
    LAIR1
  • Target description
    leukocyte associated immunoglobulin like receptor 1
  • Target synonyms
    CD305; immunoglobulin heavy chain variable region; LAIR-1; leukocyte-associated Ig-like receptor 1; leukocyte-associated immunoglobulin-like receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6GTX8
  • Protein Name
    Leukocyte-associated immunoglobulin-like receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LAIR1 using anti-LAIR1 antibody (A03470-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAIR1 antigen affinity purified polyclonal antibody (Catalog # A03470-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAIR1 at approximately 40 kDa. The expected band size for LAIR1 is at 31 kDa.
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