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Lamin B1 antibody

GTX103292
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLMNB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Lamin B1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.64 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4001
  • Target name
    LMNB1
  • Target description
    lamin B1
  • Target synonyms
    ADLD; lamin-B1; LMN; LMN2; LMNB; MCPH26
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20700
  • Protein Name
    Lamin-B1
  • Scientific Description
    The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression.
    Read more
  • Surface-exposed chaperonin 60 derived from Propionibacterium freudenreichii MJ2 inhibits adipogenesis by decreasing the expression of C/EBPalpha/PPARgamma.
    Read more
  • HPV38 impairs UV-induced transcriptional activation of the IL-18 pro-inflammatory cytokine.
    Read more
  • Anti-inflammatory effects of cold atmospheric plasma irradiation on the THP-1 human acute monocytic leukemia cell line.
    Read more
  • Lyon IARC Polyomavirus Displays Transforming Activities in Primary Human Cells.
    Read more
  • CRM1-spike-mediated nuclear export of hepatitis B virus encapsidated viral RNA. Yang CC et al., 2022 Mar 8, Cell Rep
    Read more
  • Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis. Gillotin S, 2018 Oct 5, Bio Protoc
    Read more
  • Activation of kelch-like ECH-associated protein 1/nuclear factor erythroid 2-related factor 2/antioxidant response element pathway by curcumin enhances the anti-oxidative capacity of corneal endothelial cells. Guo SP et al., 2021 Sep, Biomed Pharmacother
    Read more
  • TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells. Silva B et al., 2021 Jun 18, Nat Commun
    Read more
  • Bile Acids Elevated in Chronic Periaortitis Could Activate Farnesoid-X-Receptor to Suppress IL-6 Production by Macrophages. Cao S et al., 2021, Front Immunol
    Read more

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Figure 1. Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PB9611) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 72 kDa. The expected band size for Lamin B1 is at 66 kDa.
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