Mouse anti Lamin B1
MUB1103P-CE/IVD
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Fish, Human, Mouse, Rabbit, Rat, Sheep
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Lamin B1
- Delivery Days Customer7
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierFlow Cytometry;Immunocytochemistry;Western Blotting;Immunohistochemistry (frozen);ELISA
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- Clone ID119D5-F1
- HostMouse
- IsotypeIgG1
- Source119D5-F1 is a Mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins.
- ReactivityBovine, Canine, Fish, Human, Mouse, Rabbit, Rat, Sheep
- Reactivity SupplierBovine;Canine;Human;Mouse;Rabbit;Rat;Sheep;Zebrafish
- Storage InstructionThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
- UNSPSC12352203
References
- 1. Broers, J.L., Ramaekers, F.C.S., Bonne, G., Hutchison, C.J. (2006). Nuclear Lamins: laminopathies and their role in premature aging. Physiol Rev 86, 967-1008. 2. Weaver, V. M., Carson, C. E., Walker, P. R., Chaly, N., Lach, B., Raymond, Y., Brown, D. L., and Sikorska, M. (1996). Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes, J Cell Sci 109, 45-56. 3. Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110, 2483-93. 4. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17. 5. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. 6. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. 7. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. 8. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178, 284-95. 9. Broers, J. L., Bronnenberg, N. M., Kuijpers, H. J., Schutte, B., Hutchison, C. J., and Ramaekers, F. C. (2002). Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis. Eur J Cell Biol 81, 677-691.