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Mouse anti Lamin B1

MUB1103P-CE/IVD
Nordic-MUbio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetLMNB1
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Lamin B1
  • Delivery Days Customer
    7
  • Application Supplier Note
    119D5-F1 is suitable for immunocytochemistry on permeabilised cells, immunohistochemistry on frozen tissues, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, immunocytochemistry and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Flow Cytometry;Immunocytochemistry;Western Blotting;Immunohistochemistry (frozen);ELISA
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    119D5-F1
  • Gene ID4001
  • Target name
    LMNB1
  • Target description
    lamin B1
  • Target synonyms
    ADLD; lamin-B1; LMN; LMN2; LMNB; MCPH26
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20700
  • Protein Name
    Lamin-B1
  • Source
    119D5-F1 is a Mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins.
  • Reactivity Supplier
    Bovine;Canine;Human;Mouse;Rabbit;Rat;Sheep;Zebrafish
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PB9611) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 72 kDa. The expected band size for Lamin B1 is at 66 kDa.
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