WB (peptide competition) analysis of background extracts alone (Lane 1) or with added recombinant active human Lck protein (Lane 2-5) using GTX24900 Lck (phospho Tyr192) antibody prior incubated with the non-phosphopeptide corresponding to the non-phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4) or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of background extracts alone (Lane 1) or with added recombinant active human Lck protein (Lane 2-5) using GTX24900 Lck (phospho Tyr192) antibody prior incubated with the non-phosphopeptide corresponding to the non-phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4) or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
Lck (phospho Tyr192) antibody
GTX24900
Overview
- SupplierGeneTex
- Product NameLck (phospho Tyr192) antibody - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID3932
- Target nameLCK
- Target descriptionLCK proto-oncogene, Src family tyrosine kinase
- Target synonymsIMD22; leukocyte C-terminal Src kinase; LSK; lymphocyte cell-specific protein-tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; pp58lck; proto-oncogene tyrosine-protein kinase LCK; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16
- HostRabbit
- IsotypeIgG
- Protein IDP06239
- Protein NameTyrosine-protein kinase Lck
- Scientific DescriptionThis gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203