
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lck (phospho Tyr394) antibody (GTX133876) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Lck (phospho Tyr394) antibody
GTX133876
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLCK
Overview
- SupplierGeneTex
- Product NameLck (phospho Tyr394) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.09 mg/ml
- ConjugateUnconjugated
- Gene ID3932
- Target nameLCK
- Target descriptionLCK proto-oncogene, Src family tyrosine kinase
- Target synonymsIMD22, LSK, YT16, p56lck, pp58lck, tyrosine-protein kinase Lck, T-lymphocyte specific protein tyrosine kinase p56lck, leukocyte C-terminal Src kinase, lymphocyte cell-specific protein-tyrosine kinase, p56(LSTRA) protein-tyrosine kinase, proto-oncogene tyrosine-protein kinase LCK, t cell-specific protein-tyrosine kinase
- HostRabbit
- IsotypeIgG
- Protein IDP06239
- Protein NameTyrosine-protein kinase Lck
- Scientific DescriptionThis gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hu XX, Zhang AJ, Pan WW, et al. An IgD-Fc-Ig fusion protein restrains the activation of T and B cells by inhibiting IgD-IgDR-Lck signaling in rheumatoid arthritis. Acta Pharmacol Sin. 2022,43(2):387-400. doi: 10.1038/s41401-021-00665-wRead this paper
- He QW, Feng JH, Hu XL, et al. Synthesis and Biological Evaluation of Celastrol Derivatives as Potential Immunosuppressive Agents. J Nat Prod. 2020,83(9):2578-2586. doi: 10.1021/acs.jnatprod.0c00067Read this paper
- Duan Y, Li G, Xu M, et al. CFTR is a negative regulator of γδ T cell IFN-γ production and antitumor immunity. Cell Mol Immunol. 2021,18(8):1934-1944. doi: 10.1038/s41423-020-0499-3Read this paper
- Märklin M, Heitmann JS, Fuchs AR, et al. NFAT2 is a critical regulator of the anergic phenotype in chronic lymphocytic leukaemia. Nat Commun. 2017,8(1):755. doi: 10.1038/s41467-017-00830-yRead this paper


![Lck antibody [C1C3] detects Lck protein at cell membrane by immunofluorescent analysis. Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Lck stained by Lck antibody [C1C3] (GTX101275) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101275/GTX101275_43628_20230512_ICC_IF_23060622_378.webp)
![Lck antibody [N1C1] detects Lck protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. Lck antibody [N1C1] (GTX107432) dilution: 1:100.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107432/GTX107432_40121_CT_IHC_w_23060120_680.webp)



