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LCMT2 Polyclonal Antibody

RD218747A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLCMT2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    LCMT2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.84 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9836
  • Target name
    LCMT2
  • Target description
    leucine carboxyl methyltransferase 2
  • Target synonyms
    PPM2, TYW4, tRNA wybutosine-synthesizing protein 4, p21WAF1/CIP1 promoter-interacting protein, tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase, tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase, tRNA-Wybutosine-synthesizing protein 4, tRNA-yW synthesizing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60294
  • Protein Name
    tRNA wybutosine-synthesizing protein 4
  • Scientific Description
    The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). - This is a LCMT2 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LCMT2 using anti-LCMT2 antibody (A14797-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCMT2 antigen affinity purified polyclonal antibody (Catalog # A14797-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCMT2 at approximately 76 kDa. The expected band size for LCMT2 is at 76 kDa.
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