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Lcn2 Antibody

PACO64291
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLcn2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Lcn2 Antibody (PACO64291)
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID170496
  • Target name
    Lcn2
  • Target description
    lipocalin 2
  • Target synonyms
    Sip24, neutrophil gelatinase-associated lipocalin, NGAL, alpha-2-microglobulin-related protein, alpha-2U globulin-related protein, lipocalin 2 (oncogene 24p3), lipocalin 24p3, p25, siderocalin LCN2
  • Host
    Rabbit
  • Protein IDP30152
  • Protein Name
    Neutrophil gelatinase-associated lipocalin
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Intestine Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9609) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22KD. The expected band size for Lipocalin 2 is at 22KD.
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