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FACS analysis of A431 cells using GTX03729 Lipocalin-2 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Antibody amount : 1microg/1x106 cells
FACS analysis of A431 cells using GTX03729 Lipocalin-2 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Antibody amount : 1microg/1x106 cells
FACS analysis of A431 cells using GTX03729 Lipocalin-2 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Antibody amount : 1microg/1x106 cells

Lipocalin-2 antibody

GTX03729
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLcn2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Lipocalin-2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. IHC-P: 0.5-1microg/ml. IHC-Fr: 0.5-1microg/ml. FACS: 1-3microg/1x106 cells. ELISA: 1-5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID170496
  • Target name
    Lcn2
  • Target description
    lipocalin 2
  • Target synonyms
    alpha-2-microglobulin-related protein; alpha-2U globulin-related protein; lipocalin 2 (oncogene 24p3); lipocalin 24p3; neutrophil gelatinase-associated lipocalin; NGAL; p25; siderocalin LCN2; Sip24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30152
  • Protein Name
    Neutrophil gelatinase-associated lipocalin
  • Scientific Description
    mouse homolog plays a role in IL3 withdrawal-induced apoptosis [RGD, Feb 2006]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Intestine Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9609) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22KD. The expected band size for Lipocalin 2 is at 22KD.
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