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Neutrophil gelatinase-associated lipocalin (NGAL) Monoclonal Antibody

CAU35356
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetLcn2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Neutrophil gelatinase-associated lipocalin (NGAL) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D13
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID170496
  • Target name
    Lcn2
  • Target description
    lipocalin 2
  • Target synonyms
    alpha-2-microglobulin-related protein; alpha-2U globulin-related protein; lipocalin 2 (oncogene 24p3); lipocalin 24p3; neutrophil gelatinase-associated lipocalin; NGAL; p25; siderocalin LCN2; Sip24
  • Host
    Mouse
  • Protein IDP30152
  • Protein Name
    Neutrophil gelatinase-associated lipocalin
  • Scientific Description
    The Neutrophil gelatinase-associated lipocalin (NGAL) Monoclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Intestine Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9609) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22KD. The expected band size for Lipocalin 2 is at 22KD.
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IHC image of CSB-PA012816LA01RA diluted at 1:200 and staining in paraffin-embedded rat lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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