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LCN2 Antibody Pair

CSB-EAP06834
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetLCN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LCN2 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 3ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID3934
  • Target name
    LCN2
  • Target description
    lipocalin 2
  • Target synonyms
    24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; oncogene 24p3; p25; siderocalin LCN2
  • Host
    Mouse
  • Isotype
    IgG, IgG2b
  • Protein IDP80188
  • Protein Name
    Neutrophil gelatinase-associated lipocalin
  • Reactivity
    Human
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: A549 Whole Cell Lysate at 40ug, Lane 2: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9610) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22 kDa. The expected band size for Lipocalin 2 is at 22 kDa.
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