![IHC-P analysis of human breast adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human breast adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_2477_IHC-P_w_23061420_488.webp)
IHC-P analysis of human breast adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
LDHA antibody [2D11]
GTX84227
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLDHA
Overview
- SupplierGeneTex
- Product NameLDHA antibody [2D11] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. IHC-P: 1:50. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2D11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3939
- Target nameLDHA
- Target descriptionlactate dehydrogenase A
- Target synonymscell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; lactate dehydrogenase M; LDH muscle subunit; LDH-A; LDHM; LDH-M; L-lactate dehydrogenase A chain; PIG19; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59
- HostMouse
- IsotypeIgG1
- Protein IDP00338
- Protein NameL-lactate dehydrogenase A chain
- Scientific DescriptionThe protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_2478_IHC-P_w_23061420_342.webp)
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human ovary adenocarcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_2479_IHC-P_w_23061420_478.webp)
![IHC-P analysis of human bladder carcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human bladder carcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_2480_IHC-P_w_23061420_573.webp)
![IHC-P analysis of human pancreas carcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human pancreas carcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_2481_IHC-P_w_23061420_393.webp)
![IHC-P analysis of human thyroid carcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human thyroid carcinoma tissue using GTX84227 LDHA antibody [2D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_2482_IHC-P_w_23061420_195.webp)
![IP analysis of DDDDK tagged LDHA overexpressed HEK293T lysate using GTX84227 LDHA antibody [2D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate IP analysis of DDDDK tagged LDHA overexpressed HEK293T lysate using GTX84227 LDHA antibody [2D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_3362_IP_w_23061420_423.webp)
![WB analysis of various cell lines using GTX84227 LDHA antibody [2D11]. Loading : 35 ug per lane Dilution : 1:200 WB analysis of various cell lines using GTX84227 LDHA antibody [2D11]. Loading : 35 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_3510_WB_w_23061420_108.webp)
![WB analysis of HEK293T cells transfected with LDHA plasmid (Right) or empty vector (Left) for 48 hrs using GTX84227 LDHA antibody [2D11]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with LDHA plasmid (Right) or empty vector (Left) for 48 hrs using GTX84227 LDHA antibody [2D11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_4199_WB_w_23061420_754.webp)




