![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639069/GTX639069_T-45173_20231103_WB_multiple_B_23110819_287.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LDHA antibody [HL2615]
GTX639069
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLDHA
Overview
- SupplierGeneTex
- Product NameLDHA antibody [HL2615]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2615
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3939
- Target nameLDHA
- Target descriptionlactate dehydrogenase A
- Target synonymsGSD11, HEL-S-133P, LDHM, PIG19, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, epididymis secretory sperm binding protein Li 133P, lactate dehydrogenase M, proliferation-inducing gene 19, renal carcinoma antigen NY-REN-59
- HostRabbit
- IsotypeIgG
- Protein IDP00338
- Protein NameL-lactate dehydrogenase A chain
- Scientific DescriptionThe protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![LDHA antibody [HL2615] detects LDHA protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LDHA stained by LDHA antibody [HL2615] (GTX639069) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). LDHA antibody [HL2615] detects LDHA protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LDHA stained by LDHA antibody [HL2615] (GTX639069) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639069/GTX639069_T-45173_20231117_ICC_IF_23121122_110.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639069/GTX639069_45278_20240112_WB_24011618_125.webp)
![LDHA antibody [HL2615] detects LDHA protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LDHA stained by LDHA antibody [HL2615] (GTX639069) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min LDHA antibody [HL2615] detects LDHA protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LDHA stained by LDHA antibody [HL2615] (GTX639069) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639069/GTX639069_T-45173_20240923_IHC-P_24092600_902.webp)

![IHC-P analysis of human thyroid carcinoma tissue using GTX84226 LDHA antibody [5G10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84226/GTX84226_2476_IHC-P_w_23061420_108.webp)
![WB analysis of HEK293T cells transfected with LDHA plasmid (Right) or empty vector (Left) for 48 hrs using GTX84227 LDHA antibody [2D11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84227/GTX84227_4199_WB_w_23061420_754.webp)



