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FACS analysis of HeLa cells using GTX84221 LEMD3 antibody [3H1]. Red : Primary antibody Blue : Negative control antibody
FACS analysis of HeLa cells using GTX84221 LEMD3 antibody [3H1]. Red : Primary antibody Blue : Negative control antibody
FACS analysis of HeLa cells using GTX84221 LEMD3 antibody [3H1]. Red : Primary antibody Blue : Negative control antibody

LEMD3 antibody [3H1]

GTX84221
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetLEMD3
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Overview

  • Supplier
    GeneTex
  • Product Name
    LEMD3 antibody [3H1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100. FACS: 1:100. IP: 2-4ug/mg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23592
  • Target name
    LEMD3
  • Target description
    LEM domain containing 3
  • Target synonyms
    MAN1, inner nuclear membrane protein Man1, LEM domain-containing protein 3, LEMD3/MKX fusion, MAN antigen 1, integral inner nuclear membrane protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y2U8
  • Protein Name
    Inner nuclear membrane protein Man1
  • Scientific Description
    Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAN1/LEMD3 using anti-MAN1/LEMD3 antibody (A04957-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAN1/LEMD3 antigen affinity purified polyclonal antibody (Catalog # A04957-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAN1/LEMD3 at approximately 120 kDa. The expected band size for MAN1/LEMD3 is at 100 kDa.
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