IP analysis of DDDDK tagged LEMD3 overexpressed HEK293T lysate using GTX84222 LEMD3 antibody [3H2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
![WB analysis of various cell lines using GTX84222 LEMD3 antibody [3H2]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84222/GTX84222_4197_WB_w_23061420_474.webp "WB analysis of various cell lines using GTX84222 LEMD3 antibody [3H2]. Loading : 35 ug per lane")
![WB analysis of HEK293T cells transfected with LEMD3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84222 LEMD3 antibody [3H2]. Loading : 5 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84222/GTX84222_4455_WB_w_23061420_659.webp "WB analysis of HEK293T cells transfected with LEMD3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84222 LEMD3 antibody [3H2]. Loading : 5 ug per lane Dilution : 1:500")
IP analysis of DDDDK tagged LEMD3 overexpressed HEK293T lysate using GTX84222 LEMD3 antibody [3H2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
LEMD3 antibody [3H2] - Overexpression Validated
GTX84222
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetLEMD3
Overview
- SupplierGeneTex
- Product NameLEMD3 antibody [3H2] - Overexpression Validated
- Delivery Days Customer9
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3H2
- Concentration0.61 mg/ml
- ConjugateUnconjugated
- Gene ID23592
- Target nameLEMD3
- Target descriptionLEM domain containing 3
- Target synonymsinner nuclear membrane protein Man1; integral inner nuclear membrane protein; LEM domain-containing protein 3; LEMD3/MKX fusion; MAN antigen 1; MAN1
- HostMouse
- IsotypeIgG1
- Scientific DescriptionCan function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.
- ReactivityCanine, Human, Monkey
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203