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LGI1 Antibody (aa35-285)

LS-C376024
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLGI1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LGI1 Antibody (aa35-285)
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (1:20 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9211
  • Target name
    LGI1
  • Target description
    leucine rich glioma inactivated 1
  • Target synonyms
    ADLTE, ADPAEF, ADPEAF, EPITEMPIN, EPT, ETL1, IB1099, leucine-rich glioma-inactivated protein 1, epitempin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lgi1/EPT/LGI1 using anti-Lgi1/EPT/LGI1 antibody (A00850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lgi1/EPT/LGI1 antigen affinity purified polyclonal antibody (Catalog # A00850-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lgi1/EPT/LGI1 at approximately 64KD. The expected band size for Lgi1/EPT/LGI1 is at 64KD.
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