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Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using LILRB1 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using LILRB1 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using LILRB1 Polyclonal Antibody at dilution 1:50

LILRB1 Polyclonal Antibody

E-AB-10412
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    LILRB1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LILRB1 using anti-LILRB1 antibody (A03208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LILRB1 antigen affinity purified polyclonal antibody (Catalog # A03208-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LILRB1 at approximately 71 kDa. The expected band size for LILRB1 is at 71 kDa.
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