Bio-Connect

LIMS1 antibody [N1C2]

GTX114984
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLIMS1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    LIMS1 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.88 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3987
  • Target name
    LIMS1
  • Target description
    LIM zinc finger domain containing 1
  • Target synonyms
    LIM and senescent cell antigen-like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIM-type zinc finger domains 1; particularly interesting new Cys-His protein 1; PINCH; PINCH1; PINCH-1; renal carcinoma antigen NY-REN-48; senescent cell antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48059
  • Protein Name
    LIM and senescent cell antigen-like-containing domain protein 1
  • Scientific Description
    The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetLIMS1
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetLIMS1
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetLIMS1
  • SizePrice
Figure 1. Western blot analysis of LIMS1 using anti-LIMS1 antibody (A04072-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMS1 antigen affinity purified polyclonal antibody (Catalog # A04072-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMS1 at approximately 37 kDa. The expected band size for LIMS1 is at 37 kDa.
Product group Antibodies
Boster Bio
Anti-LIMS1 Antibody Picoband(r)A04072-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetLIMS1
  • SizePrice
Anti-LIMS1 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetLIMS1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetLIMS1
  • SizePrice
Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetLIMS1
  • SizePrice
ELISA analysis of antigen using GTX60472 LIMS1 antibody [5G7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetLIMS1
  • SizePrice
Western Blot Positive WB detected in: Rat kidney tissue All lanes: LIMS1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 39, 45, 42 kDa Observed band size: 38 kDa
Product group Antibodies
Cusabio
LIMS1 AntibodyCSB-PA012955EA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetLIMS1
  • SizePrice