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ProductsBack/LMAN1 Mouse Monoclonal Antibody [Clone ID: OTI1A8]
Simple Western™ analysis of endogenous protein LMAN1 from HEK293T lysates (0.2 mg/mL) using LMAN1 Mouse Monoclonal Antibody #TA502137. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein LMAN1 from HEK293T lysates (0.2 mg/mL) using LMAN1 Mouse Monoclonal Antibody #TA502137. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein LMAN1 from HEK293T lysates (0.2 mg/mL) using LMAN1 Mouse Monoclonal Antibody #TA502137. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

LMAN1 Mouse Monoclonal Antibody [Clone ID: OTI1A8]

CF502137
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetLMAN1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    LMAN1 Mouse Monoclonal Antibody [Clone ID: OTI1A8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1A8
  • Gene ID3998
  • Target name
    LMAN1
  • Target description
    lectin, mannose binding 1
  • Target synonyms
    ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58, protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, endoplasmic reticulum-golgi intermediate compartment protein 53, intracellular mannose-specific lectin MR60
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP49257
  • Protein Name
    Protein ERGIC-53
  • Scientific Description
    Carrier-free (BSA/glycerol-free) LMAN1 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody (Catalog # A03628-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58 kDa. The expected band size for LMAN1 is at 58 kDa.
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