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LMO7 Antibody

PACO60236
Assay Genie
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetLMO7
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Overview

  • Supplier
    Assay Genie
  • Product Name
    LMO7 Antibody (PACO60236)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4008
  • Target name
    LMO7
  • Target description
    LIM domain 7
  • Target synonyms
    F-box only protein 20; F-box protein Fbx20; FBX20; FBXO20; LIM domain only 7 protein; LIM domain only protein 7; LMO-7; LMO7b; LOMP; zinc-finger domain-containing protein
  • Host
    Rabbit
  • Protein IDQ8WWI1
  • Protein Name
    LIM domain only protein 7
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LMO7 using anti-LMO7 antibody (A05712-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO7 antigen affinity purified polyclonal antibody (Catalog # A05712-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO7 at approximately 150 kDa. The expected band size for LMO7 is at 193 kDa.
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