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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA013032LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA013032LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA013032LA01HU at dilution of 1:100

LONP1 Antibody

CSB-PA013032LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLONP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LONP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9361
  • Target name
    LONP1
  • Target description
    lon peptidase 1, mitochondrial
  • Target synonyms
    CODASS; hLON; hLON ATP-dependent protease; LON; lon protease homolog, mitochondrial; LonHS; LONP; mitochondrial ATP-dependent protease Lon; mitochondrial lon protease-like protein; PIM1; PRSS15; serine protease 15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36776
  • Protein Name
    Lon protease homolog, mitochondrial
  • Scientific Description
    ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters. Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LONP1 using anti-LONP1 antibody (A03808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human U-87MG whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human A549 whole cell lysates Lane 5: mouse liver tissue lysates Lane 6: mouse kidney tissue lysates Lane 7: mouse SP20 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONP1 antigen affinity purified polyclonal antibody (Catalog # A03808-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONP1 at approximately 106KD. The expected band size for LONP1 is at 106KD.
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