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Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

LONP1 Polyclonal Antibody

BS-4245R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat
TargetLONP1
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Overview

  • Supplier
    Bioss
  • Product Name
    LONP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9361
  • Target name
    LONP1
  • Target description
    lon peptidase 1, mitochondrial
  • Target synonyms
    CODASS, LON, LONP, LonHS, PIM1, PRSS15, hLON, lon protease homolog, mitochondrial, hLON ATP-dependent protease, mitochondrial ATP-dependent protease Lon, mitochondrial lon protease-like protein, serine protease 15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36776
  • Protein Name
    Lon protease homolog, mitochondrial
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Nie X, Li M, Lu B, et al. Down-regulating overexpressed human Lon in cervical cancer suppresses cell proliferation and bioenergetics. PLoS One. 2013,8(11):e81084. doi: 10.1371/journal.pone.0081084
    Read this paper

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Figure 1. Western blot analysis of LONP1 using anti-LONP1 antibody (A03808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human U-87MG whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human A549 whole cell lysates Lane 5: mouse liver tissue lysates Lane 6: mouse kidney tissue lysates Lane 7: mouse SP20 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONP1 antigen affinity purified polyclonal antibody (Catalog # A03808-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONP1 at approximately 106KD. The expected band size for LONP1 is at 106KD.
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