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LONP1 / LON Antibody

LS-C667905
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetLONP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LONP1 / LON Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of LONP1 expression in A549 (A), mouse heart (B), rat kidney (C) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of LONP1 expression in A549 (A), mouse heart (B), rat kidney (C) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9361
  • Target name
    LONP1
  • Target description
    lon peptidase 1, mitochondrial
  • Target synonyms
    CODASS, LON, LONP, LonHS, PIM1, PRSS15, hLON, lon protease homolog, mitochondrial, hLON ATP-dependent protease, mitochondrial ATP-dependent protease Lon, mitochondrial lon protease-like protein, serine protease 15
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LONP1 using anti-LONP1 antibody (A03808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human U-87MG whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human A549 whole cell lysates Lane 5: mouse liver tissue lysates Lane 6: mouse kidney tissue lysates Lane 7: mouse SP20 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONP1 antigen affinity purified polyclonal antibody (Catalog # A03808-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONP1 at approximately 106KD. The expected band size for LONP1 is at 106KD.
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