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LOXL2 antibody

GTX105085
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLOXL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    LOXL2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.02 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4017
  • Target name
    LOXL2
  • Target description
    lysyl oxidase like 2
  • Target synonyms
    LOR; LOR2; lysyl oxidase homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2 delta e13; lysyl oxidase-like 2 protein; lysyl oxidase-like protein 2; lysyl oxidase-related protein 2; lysyl oxidase-related protein WS9-14; WS9-14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4K0
  • Protein Name
    Lysyl oxidase homolog 2
  • Scientific Description
    This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Pharmacological targeting of the mitochondrial calcium-dependent potassium channel KCa3.1 triggers cell death and reduces tumor growth and metastasis in vivo.
    Read more
  • Reciprocal regulation of LOXL2 and HIF1alpha drives the Warburg effect to support pancreatic cancer aggressiveness. Li R et al., 2021 Nov 26, Cell Death Dis
    Read more
  • The Expression Patterns and Roles of Lysyl Oxidases in Aortic Dissection. Yi X et al., 2021, Front Cardiovasc Med
    Read more
  • MIR29A Impedes Metastatic Behaviors in Hepatocellular Carcinoma via Targeting LOX, LOXL2, and VEGFA. Yang YL et al., 2021 Jun 1, Int J Mol Sci
    Read more
  • Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma. Yang K et al., 2021 Jan 11, Cell Div
    Read more
  • LOXL2 promotes aggrecan and gender-specific anabolic differences to TMJ cartilage. Tashkandi MM et al., 2020 Nov 19, Sci Rep
    Read more
  • Mechanical injury and IL-1beta regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes. Wang C et al., 2020 Aug, Biotechnol Lett
    Read more
  • LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) Phenotype. Cao C et al., 2020, Front Oncol
    Read more
  • Withaferin A reverses bile duct ligation-induced liver fibrosis by modulating extracellular matrix deposition: Role of LOXL2/Snail1, vimentin, and NFkappaB signaling. Sayed N et al., 2019 Jul 23, Biofactors
    Read more
  • Mechanism for oral tumor cell lysyl oxidase like-2 in cancer development: synergy with PDGF-AB. Mahjour F et al., 2019 May 13, Oncogenesis
    Read more

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Figure 1. Western blot analysis of LOXL2 using anti-LOXL2 antibody (PB9720). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL2 antigen affinity purified polyclonal antibody (Catalog # PB9720) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL2 at approximately 105 kDa. The expected band size for LOXL2 is at 87 kDa.
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