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LOXL2 antibody

70R-12876
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    LOXL2 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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LOXL2 antibody detects LOXL2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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Lane 1: PC-12 Cells; Lane 2: A549 Cells; Probed with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of LOXL2 using anti-LOXL2 antibody (PB9720). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL2 antigen affinity purified polyclonal antibody (Catalog # PB9720) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL2 at approximately 105 kDa. The expected band size for LOXL2 is at 87 kDa.
Product group Antibodies
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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
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