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ProductsBack/LOXL2 Recombinant Antibody
Lane 1: PC-12 Cells; Lane 2: A549 Cells; Probed with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: PC-12 Cells; Lane 2: A549 Cells; Probed with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: PC-12 Cells; Lane 2: A549 Cells; Probed with LOXL2 (7D7) Monoclonal Antibody (bsm-54163R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

LOXL2 Recombinant Antibody

BSM-54163R
Bioss Antibodies
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetLOXL2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    LOXL2 (7D7) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4017
  • Target name
    LOXL2
  • Target description
    lysyl oxidase like 2
  • Target synonyms
    LOR; LOR2; lysyl oxidase homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2 delta e13; lysyl oxidase-like 2 protein; lysyl oxidase-like protein 2; lysyl oxidase-related protein 2; lysyl oxidase-related protein WS9-14; WS9-14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4K0
  • Protein Name
    Lysyl oxidase homolog 2
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Rat lung tissue, Rat liver tissue, Mouse heart tissue, Mouse lung tissue All lanes: LOXL2 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87 kDa Observed band size: 87 kDa
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Figure 1. Western blot analysis of LOXL2 using anti-LOXL2 antibody (PB9720). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL2 antigen affinity purified polyclonal antibody (Catalog # PB9720) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL2 at approximately 105 kDa. The expected band size for LOXL2 is at 87 kDa.
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