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ProductsBack/Rabbit anti Human Lysophosphatidic Acid Receptor 3 CT (EDG-7)

Rabbit anti Human Lysophosphatidic Acid Receptor 3 CT (EDG-7)

X1183P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetLPAR3
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Lysophosphatidic Acid Receptor 3 CT (EDG-7)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Detects recombinant EDG-7 receptors by Western blot. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23566
  • Target name
    LPAR3
  • Target description
    lysophosphatidic acid receptor 3
  • Target synonyms
    calcium-mobilizing lysophosphatidic acid receptor LP-A3; EDG7; Edg-7; endothelial cell differentiation gene 7; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7; GPCR; HOFNH30; LPA receptor 3; LPA receptor EDG7; LPA3; LP-A3; LPA-3; lysophosphatidic acid receptor 3; lysophosphatidic acid receptor Edg-7; RP4-678I3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBY5
  • Protein Name
    Lysophosphatidic acid receptor 3
  • Scientific Description
    Endothelial cell differentiation gene 7 C-terminal; Lysophosphatidic Acid Receptor-4 (LPA3)
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Antibody raised against a unique synthetic peptide derived from the C-terminal of the human EDG7 protein.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDG7/LPAR3 using anti-EDG7/LPAR3 antibody (A06597-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG7/LPAR3 antigen affinity purified polyclonal antibody (Catalog # A06597-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG7/LPAR3 at approximately 43 kDa. The expected band size for EDG7/LPAR3 is at 40 kDa.
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