IHC-P analysis of human ovary adenocarcinoma tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
![IHC-P analysis of prostate carcinoma tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_2424_IHC-P_w_23061420_915.webp "IHC-P analysis of prostate carcinoma tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of kidney tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_2425_IHC-P_w_23061420_518.webp "IHC-P analysis of kidney tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of liver tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_2426_IHC-P_w_23061420_313.webp "IHC-P analysis of liver tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of prostate tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_2427_IHC-P_w_23061420_498.webp "IHC-P analysis of prostate tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![FACS analysis of HEK293T cells transfected with either LTA4H plasmid(Red) or empty vector control plasmid(Blue) using GTX84173 LTA4H antibody [6B5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_318_FACS_w_23061420_833.webp "FACS analysis of HEK293T cells transfected with either LTA4H plasmid(Red) or empty vector control plasmid(Blue) using GTX84173 LTA4H antibody [6B5].")
![IP analysis of DDDDK tagged LTA4H overexpressed HEK293T lysate using GTX84173 LTA4H antibody [6B5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_3367_IP_w_23061420_561.webp "IP analysis of DDDDK tagged LTA4H overexpressed HEK293T lysate using GTX84173 LTA4H antibody [6B5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of various cell lines using GTX84173 LTA4H antibody [6B5]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_3500_WB_w_23061420_956.webp "WB analysis of various cell lines using GTX84173 LTA4H antibody [6B5]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of wild type and LTA4H knock out 293T cell lysate(10microg per lane) using GTX84173 LTA4H antibody [6B5]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_4180_20181228_WB_w_23061420_303.webp "WB analysis of wild type and LTA4H knock out 293T cell lysate(10microg per lane) using GTX84173 LTA4H antibody [6B5]. Dilution : 1:500")
![WB analysis of HEK293T cells transfected with LTA4H plasmid (Right) or empty vector (Left) for 48 hrs using GTX84173 LTA4H antibody [6B5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84173/GTX84173_4181_WB_w_23061420_638.webp "WB analysis of HEK293T cells transfected with LTA4H plasmid (Right) or empty vector (Left) for 48 hrs using GTX84173 LTA4H antibody [6B5]. Loading : 5 ug per lane")
IHC-P analysis of human ovary adenocarcinoma tissue using GTX84173 LTA4H antibody [6B5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
LTA4H antibody [6B5]
GTX84173
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetLTA4H
Overview
- SupplierGeneTex
- Product NameLTA4H antibody [6B5] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. IHC-P: 1:50. FACS: 1:100. IP: 2-4ug/mg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6B5
- Concentration0.75 mg/ml
- ConjugateUnconjugated
- Gene ID4048
- Target nameLTA4H
- Target descriptionleukotriene A4 hydrolase
- Target synonymsleukotriene A-4 hydrolase; LTA-4 hydrolase; testicular secretory protein Li 27; tripeptide aminopeptidase LTA4H
- HostMouse
- IsotypeIgG2b
- Protein IDP09960
- Protein NameLeukotriene A-4 hydrolase
- Scientific DescriptionHydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity.
- ReactivityCanine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Distinct compartmentalization of immune cells and mediators characterizes bullous pemphigoid disease. Margaroli C et al., 2020 Dec, Exp DermatolRead more