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Lysozyme antibody

GTX72913
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLYZ
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Overview

  • Supplier
    GeneTex
  • Product Name
    Lysozyme antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4069
  • Target name
    LYZ
  • Target description
    lysozyme
  • Target synonyms
    AMYLD5, LYZF1, LZM, lysozyme C, 1,4-beta-N-acetylmuramidase C, c-type lysozyme, lysozyme F1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61626
  • Protein Name
    Lysozyme C
  • Scientific Description
    This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Kanno T, Katano T, Ogawa I, et al. Protective Effect of Irsogladine against Aspirin-Induced Mucosal Injury in Human Induced Pluripotent Stem Cell-Derived Small Intestine. Medicina (Kaunas). 2022,59(1). doi: 10.3390/medicina59010092
    Read this paper
  • Zwiggelaar RT, Lindholm HT, Fosslie M, et al. LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium. Sci Adv. 2020,6(37). doi: 10.1126/sciadv.abc0367
    Read this paper
  • Fujii M, Matano M, Toshimitsu K, et al. Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition. Cell Stem Cell. 2018,23(6):787-793.e6. doi: 10.1016/j.stem.2018.11.016
    Read this paper
  • Otsuka K, Suzuki K. Differences in Radiation Dose Response between Small and Large Intestinal Crypts. Radiat Res. 2016,186(3):302-14. doi: 10.1667/RR14455.1
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (A01811-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (Catalog # A01811-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme/LYZ at approximately 15-17 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.
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