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Immunofluorescence staining of HepG2 with CSB-RA949150A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 505-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 with CSB-RA949150A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 505-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 with CSB-RA949150A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 505-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

LYVE1 Recombinant Monoclonal Antibody

CSB-RA949150A0HU
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetLYVE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LYVE1 Recombinant Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11C11
  • Conjugate
    Unconjugated
  • Gene ID10894
  • Target name
    LYVE1
  • Target description
    lymphatic vessel endothelial hyaluronan receptor 1
  • Target synonyms
    CRSBP-1, HAR, LYVE-1, XLKD1, lymphatic vessel endothelial hyaluronic acid receptor 1, cell surface retention sequence binding protein-1, extracellular link domain-containing 1, extracellular link domain-containing protein 1, hyaluronic acid receptor
  • Isotype
    IgG
  • Protein IDQ9Y5Y7
  • Protein Name
    Lymphatic vessel endothelial hyaluronic acid receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LYVE1 using anti-LYVE1 antibody (M04027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYVE1 antigen affinity purified monoclonal antibody (Catalog # M04027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYVE1 at approximately 29 kDa. The expected band size for LYVE1 is at 35 kDa.
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYVE1 antibody (GTX133081) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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