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Western Blot analysis of Hela, 231, hepG2 and Raji cell using MAD1L1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela, 231, hepG2 and Raji cell using MAD1L1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela, 231, hepG2 and Raji cell using MAD1L1 Polyclonal Antibody at dilution of 1:500

MAD1L1 Polyclonal Antibody

E-AB-10440
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetMAD1L1
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Overview

  • Supplier
    Elabscience
  • Product Name
    MAD1L1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8379
  • Target name
    MAD1L1
  • Target description
    mitotic arrest deficient 1 like 1
  • Target synonyms
    MAD1, MVA7, PIG9, TP53I9, TXBP181, mitotic spindle assembly checkpoint protein MAD1, MAD1 mitotic arrest deficient like 1, MAD1-like protein 1, mitotic arrest deficient 1-like protein 1, mitotic checkpoint MAD1 protein homolog, mitotic-arrest deficient 1, yeast, homolog-like 1, tax-binding protein 181, tumor protein p53 inducible protein 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6D9
  • Protein Name
    Mitotic spindle assembly checkpoint protein MAD1
  • Scientific Description
    MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts witHuman, MouseAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAD1 using anti-MAD1 antibody (PB9262). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. Lane 5: human HL-60 whole cell lysates. Lane 6: human THP-1 whole cell lysates. Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody (Catalog # PB9262) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
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