Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.
![MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX104680/GTX104680_39638_20151227_IHC-P_w_23060120_799.webp "MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104680/GTX104680_39638_20150407_IP_w_23060120_783.webp "Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX104680/GTX104680_39638_IHC_R_w_23060120_224.webp "MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104680/GTX104680_39638_20160818_WB_shRNA_watermark_w_23060120_723.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104680/GTX104680_44133_20201113_WB_24120522_478.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.
MAD2L1 antibody [C2C3], C-term
GTX104680
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetMAD2L1
Overview
- SupplierGeneTex
- Product NameMAD2L1 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.12 mg/ml
- ConjugateUnconjugated
- Gene ID4085
- Target nameMAD2L1
- Target descriptionmitotic arrest deficient 2 like 1
- Target synonymsHSMAD2, MAD2, mitotic spindle assembly checkpoint protein MAD2A, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2 mitotic arrest deficient-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1, mitotic arrest deficient, yeast, homolog-like 1
- HostRabbit
- IsotypeIgG
- Protein IDQ13257
- Protein NameMitotic spindle assembly checkpoint protein MAD2A
- Scientific DescriptionMAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wu YH, Yang Y, Chen CH, et al. Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2. PLoS Pathog. 2021,17(3):e1008866. doi: 10.1371/journal.ppat.1008866Read this paper
- Jerabkova K, Liao Y, Kleiss C, et al. Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis. FASEB J. 2020,34(9):12751-12767. doi: 10.1096/fj.202000769RRead this paper
- Chen Y, Yeh PC, Huang JC, et al. The spindle checkpoint protein MAD1 regulates the expression of E-cadherin and prevents cell migration. Oncol Rep. 2012,27(2):487-91. doi: 10.3892/or.2011.1519Read this paper
Datasheet
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