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SMAD7 antibody

GTX134232
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
DownloadDatasheet
Product group Antibodies
TargetSMAD7
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Overview

  • Supplier
    GeneTex
  • Product Name
    MADH7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.34 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4092
  • Target name
    SMAD7
  • Target description
    SMAD family member 7
  • Target synonyms
    CRCS3; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; MAD homolog 8; MADH7; MADH8; mothers against decapentaplegic homolog 7; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 8; SMAD, mothers against DPP homolog 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15105
  • Protein Name
    Mothers against decapentaplegic homolog 7
  • Scientific Description
    The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MADH7/SMAD7 using anti-MADH7/SMAD7 antibody (A00784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADH7/SMAD7 antigen affinity purified polyclonal antibody (Catalog # A00784-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADH7/SMAD7 at approximately 50KD. The expected band size for MADH7/SMAD7 is at 50KD.
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