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Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad7 Polyclonal Antibody, Unconjugated (bs-0566R) at 1:600 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad7 Polyclonal Antibody, Unconjugated (bs-0566R) at 1:600 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad7 Polyclonal Antibody, Unconjugated (bs-0566R) at 1:600 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Smad7 Polyclonal Antibody

BS-0566R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSMAD7
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Overview

  • Supplier
    Bioss
  • Product Name
    Smad7 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4092
  • Target name
    SMAD7
  • Target description
    SMAD family member 7
  • Target synonyms
    CRCS3, MADH7, MADH8, mothers against decapentaplegic homolog 7, MAD (mothers against decapentaplegic, Drosophila) homolog 7, MAD homolog 8, Mothers against decapentaplegic, drosophila, homolog of, 7, SMAD, mothers against DPP homolog 7, hSMAD7, mothers against DPP homolog 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15105
  • Protein Name
    Mothers against decapentaplegic homolog 7
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zheng HX, Qi SS, He J, et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020,68(15):4399-4410. doi: 10.1021/acs.jafc.0c00680
    Read this paper
  • Yang Y, Ding S, Xu G, et al. MicroRNA-15a inhibition protects against hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by targeting mothers against decapentaplegic homolog 7. Mol Med Rep. 2017,15(6):3699-3705. doi: 10.3892/mmr.2017.6466
    Read this paper
  • Pabón MM, Acosta S, Guedes VA, et al. Brain Region-Specific Histopathological Effects of Varying Trajectories of Controlled Cortical Impact Injury Model of Traumatic Brain Injury. CNS Neurosci Ther. 2016,22(3):200-11. doi: 10.1111/cns.12485
    Read this paper

Datasheet

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