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Western Blot Positive WB detected in: HepG2 whole cell lysate, LO2 whole cell lysate All lanes: SMAD7 antibody at 2.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 24 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, LO2 whole cell lysate All lanes: SMAD7 antibody at 2.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 24 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, LO2 whole cell lysate All lanes: SMAD7 antibody at 2.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 24 kDa Observed band size: 47 kDa

SMAD7 Antibody

CSB-PA021793LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSMAD7
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMAD7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4092
  • Target name
    SMAD7
  • Target description
    SMAD family member 7
  • Target synonyms
    CRCS3; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; MAD homolog 8; MADH7; MADH8; mothers against decapentaplegic homolog 7; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 8; SMAD, mothers against DPP homolog 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15105
  • Protein Name
    Mothers against decapentaplegic homolog 7
  • Scientific Description
    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MADH7/SMAD7 using anti-MADH7/SMAD7 antibody (A00784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADH7/SMAD7 antigen affinity purified polyclonal antibody (Catalog # A00784-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADH7/SMAD7 at approximately 50KD. The expected band size for MADH7/SMAD7 is at 50KD.
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