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ProductsBack/MAP2K1 Mouse Monoclonal Antibody [Clone ID: 13B6.G12]
Western Blot of Anti-MEK1 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50ng per lane. Primary Antibody: Anti-MEK1 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40,000 dilution.
Western Blot of Anti-MEK1 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50ng per lane. Primary Antibody: Anti-MEK1 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40,000 dilution.
Western Blot of Anti-MEK1 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50ng per lane. Primary Antibody: Anti-MEK1 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40,000 dilution.

MAP2K1 Mouse Monoclonal Antibody [Clone ID: 13B6.G12]

TA396832
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MAP2K1 Mouse Monoclonal Antibody [Clone ID: 13B6.G12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    13B6.G12
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1, dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    MEK1 C-Term Antibody HRP
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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