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ProductsBack/MAP2K1 Rabbit Polyclonal Antibody
WB: The whole cell lysates derived from Starved 3T3 were immunoblotted by Rabbit antiMEK1/2 (pSer218/222) at 1:1000 (lane 1). The lane 2 was a negative control.
WB: The whole cell lysates derived from Starved 3T3 were immunoblotted by Rabbit antiMEK1/2 (pSer218/222) at 1:1000 (lane 1). The lane 2 was a negative control.
WB: The whole cell lysates derived from Starved 3T3 were immunoblotted by Rabbit antiMEK1/2 (pSer218/222) at 1:1000 (lane 1). The lane 2 was a negative control.

MAP2K1 Rabbit Polyclonal Antibody

TA354833
OriGene
ApplicationsDot Blot, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MAP2K1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Dot Blot, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit anti MEK1/2(pS218/S222) Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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