MAP3K5 / ASK1 Antibody (phospho-Ser83)

LifeSpan BioSciences

MAP3K5 / ASK1 Antibody (phospho-Ser83)

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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.

This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. A 155 kD band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control.. ELISA (1:5000 - 1:10000), WB (1:1000) This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. A 155 kD band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control.

Western Blot, ELISA

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

MAP3K5

mitogen-activated protein kinase kinase kinase 5

apoptosis signal-regulating kinase 1; ASK1; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; mitogen-activated protein kinase kinase kinase 5

Rabbit

IgG

Phosphorylated

Human

-20°C

12352203