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ProductsBack/MAP3K8 Antibody - N-terminal region (ARP30650_P050)

MAP3K8 Antibody - N-terminal region (ARP30650_P050)

ARP30650_P050
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetMAP3K8
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    MAP3K8 Antibody - N-terminal region (ARP30650_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID1326
  • Target name
    MAP3K8
  • Target description
    mitogen-activated protein kinase kinase kinase 8
  • Target synonyms
    AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT, mitogen-activated protein kinase kinase kinase 8, Ewing sarcoma transformant, augmented in rheumatoid arthritis 2, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K8 antigen affinity purified polyclonal antibody (Catalog # PB10007) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K8 at approximately 53KD. The expected band size for MAP3K8 is at 53KD.
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