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Western blot analysis of CD45 using anti-CD45 antibody (PB9785). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Whole Cell Lysate, Lane 2: HL-60 Whole Cell Lysate, Lane 3: K562 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45 antigen affinity purified polyclonal antibody (Catalog # PB9785) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45 at approximately 220KD. The expected band size for CD45 is at 220KD.
Product group Antibodies
Boster Bio
Anti-CD45/PTPRC Antibody Picoband(r)PB9785
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPTPRC
IHC analysis of LAR using anti-LAR antibody (PB9786).
Product group Antibodies
Boster Bio
Anti-LAR/PTPRF Antibody Picoband(r)PB9786
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPTPRF
Western blot analysis of Rab5 using anti-Rab5 antibody (PB9787).
Product group Antibodies
Boster Bio
Anti-Rab5/RAB5A Antibody Picoband(r)PB9787
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAB5A
Western blot analysis of RAB10 using anti-RAB10 antibody (PB9788).
Product group Antibodies
Boster Bio
Anti-RAB10 Antibody Picoband(r)PB9788
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetRAB10
IF analysis of Rab11A using anti-Rab11A antibody (PB9789) Rab11A was detected in immunocytochemical section of human U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Rab11A Antibody (PB9789) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Product group Antibodies
Boster Bio
Anti-Rab11A Antibody Picoband(r)PB9789
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetRAB11A
Western blot analysis of RAB13 using anti-RAB13 antibody (PB9790).
Product group Antibodies
Boster Bio
Anti-RAB13 Antibody Picoband(r)PB9790
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human
TargetRAB13
Western blot analysis of ADAM10 using anti-ADAM10 antibody (A00566-1).
Product group Antibodies
Boster Bio
Anti-ADAM10 Antibody Picoband(r)A00566-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetADAM10
Western blot analysis of HLA Class II DRB1 using anti-HLA Class II DRB1 antibody (A00568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysate, Lane 2: human Raji whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA Class II DRB1 antigen affinity purified polyclonal antibody (Catalog # A00568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA Class II DRB1 at approximately 30KD. The expected band size for HLA Class II DRB1 is at 30KD.
Product group Antibodies
Boster Bio
Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband(r)A00568
ApplicationsWestern Blot
ReactivityHuman
TargetHLA-DRB1
Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1).
Product group Antibodies
Boster Bio
Anti-GSTM1 Antibody Picoband(r)A00569-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetGSTM1
IHC analysis of XRCC1 using anti-XRCC1 antibody (A00571).
Product group Antibodies
Boster Bio
Anti-XRCC1 Antibody Picoband(r)A00571
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetXRCC1
Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (A00576-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody (Catalog # A00576-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
Product group Antibodies
Boster Bio
Anti-TSH Receptor/TSHR Antibody Picoband(r)A00576-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTSHR
Western blot analysis of Eph receptor A2 using anti-Eph receptor A2 antibody (A00578).
Product group Antibodies
Boster Bio
Anti-Eph receptor A2/EPHA2 Antibody Picoband(r)A00578
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetEPHA2
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