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Western blot analysis of Desmoplakin using anti-Desmoplakin antibody (A00616-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoplakin antigen affinity purified polyclonal antibody (Catalog # A00616-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoplakin at approximately 250-280KD. The expected band size for Desmoplakin is at 332KD.
Product group Antibodies
Boster Bio
Anti-Desmoplakin/DSP Antibody Picoband(r)A00616-1
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDSP
Flow Cytometry analysis of SiHa cells using anti-MR1 antibody (A00618-1). Overlay histogram showing SiHa cells stained with A00618-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MR1 Antibody (A00618-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-MR1 Antibody Picoband(r)A00618-1
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMR1
Western blot analysis of CSF1 using anti-CSF1 antibody (A00620-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF1 antigen affinity purified polyclonal antibody (Catalog # A00620-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF1 at approximately 60KD. The expected band size for CSF1 is at 60KD.
Product group Antibodies
Boster Bio
Anti-CSF1 Antibody Picoband(r)A00620-3
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetCSF1
Western blot analysis of ATTY using anti-ATTY antibody (A00622).
Product group Antibodies
Boster Bio
Anti-ATTY/TAT Antibody Picoband(r)A00622
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTAT
Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625).
Product group Antibodies
Boster Bio
Anti-TRPC6 Antibody Picoband(r)A00625
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTRPC6
Western blot analysis of DKK1 using anti-DKK1 antibody (A00632). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human DKK1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # A00632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 38KD. The expected band size for DKK1 is at 26KD.
Product group Antibodies
Boster Bio
Anti-DKK1 Antibody Picoband(r)A00632
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetDKK1
IHC analysis of Lactoferrin using anti-Lactoferrin antibody (A00633-1).
Product group Antibodies
Boster Bio
Anti-Lactoferrin/LTF Antibody Picoband(r)A00633-1
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLTF
Western blot analysis of PAI1 using anti-PAI1 antibody (A00637-2).
Product group Antibodies
Boster Bio
Anti-PAI1/Serpine1 Antibody Picoband(r)A00637-2
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetSERPINE1
IHC analysis of MVP using anti-MVP antibody (A00642-1).
Product group Antibodies
Boster Bio
Anti-MVP Antibody Picoband(r)A00642-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetMVP
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human MVP DyLight(r) 488 conjugated AntibodyA00642-Dyl488
ApplicationsFlow Cytometry
ReactivityHuman
TargetMVP
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human MVP DyLight(r) 550 conjugated AntibodyA00642-Dyl550
ApplicationsFlow Cytometry
ReactivityHuman
TargetMVP
Western blot analysis of TRF2 using anti-TRF2 antibody (A00650-1).
Product group Antibodies
Boster Bio
Anti-TRF2/TERF2 Antibody Picoband(r)A00650-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetTERF2
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