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Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-1).
Product group Antibodies
Boster Bio
Anti-PTPN22 Antibody Picoband(r)A00581-1
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPtpn22
IHC analysis of NSF using anti-NSF antibody (A00585).
Product group Antibodies
Boster Bio
Anti-NSF Antibody Picoband(r)A00585
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNSF
Western blot analysis of ADAM17 using anti-ADAM17 antibody (A00604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody (Catalog # A00604) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 78KD. The expected band size for ADAM17 is at 97KD.
Product group Antibodies
Boster Bio
Anti-ADAM17 Antibody Picoband(r)A00604
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetADAM17
Western blot analysis of Galanin using anti-Galanin antibody (A00606-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galanin antigen affinity purified polyclonal antibody (Catalog # A00606-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galanin at approximately 19KD. The expected band size for Galanin is at 13KD.
Product group Antibodies
References
Boster Bio
Anti-Galanin Antibody Picoband(r)A00606-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGAL
Western blot analysis of IL23 Receptor using anti-IL23 Receptor antibody (A00607-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 Receptor antigen affinity purified polyclonal antibody (Catalog # A00607-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 Receptor at approximately 58KD. The expected band size for IL23 Receptor is at 72KD.
Product group Antibodies
Boster Bio
Anti-IL23 Receptor/Il23r Antibody Picoband(r)A00607-1
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetIl23r
Western blot analysis of GAS 6 using anti-GAS 6 antibody (A00608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS 6 antigen affinity purified polyclonal antibody (Catalog # A00608-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS 6 at approximately 75KD. The expected band size for GAS 6 is at 79KD.
Product group Antibodies
Boster Bio
Anti-GAS 6/GAS6 Antibody Picoband(r)A00608-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAS6
IHC analysis of PTP1B using anti-PTP1B antibody (A00613-1). PTP1B was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTP1B Antibody (A00613-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-PTP1B/PTPN1 Antibody Picoband(r)A00613-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetPTPN1
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human PTP1B DyLight(r) 488 conjugated PTPN1 AntibodyA00613-Dyl488
ApplicationsFlow Cytometry
ReactivityHuman
TargetPTPN1
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human PTP1B DyLight(r) 550 conjugated PTPN1 AntibodyA00613-Dyl550
ApplicationsFlow Cytometry
ReactivityHuman
TargetPTPN1
Western blot analysis of SRY using anti-SRY antibody (A00614-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRY antigen affinity purified polyclonal antibody (Catalog # A00614-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRY at approximately 26KD. The expected band size for SRY is at 24KD.
Product group Antibodies
Boster Bio
Anti-SRY Antibody Picoband(r)A00614-1
ApplicationsWestern Blot
ReactivityHuman
TargetSRY
Western blot analysis of CYP17A1 using anti-CYP17A1 antibody (A00615-1).
Product group Antibodies
Boster Bio
Anti-Cytochrome P450 17A1/CYP17A1 Antibody Picoband(r)A00615-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetCYP17A1
IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3). Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband(r)A00615-3
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCyp17a1
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