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Western blot analysis of SLC26A4 using anti-SLC26A4 antibody (A00919-1).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSLC26A4
IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922-1). IGFBP1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGFBP1 Antibody (A00922-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetIgfbp1
IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetIGFBP1
Western blot analysis of EML4 using anti-EML4 antibody (A00930-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EML4 antigen affinity purified polyclonal antibody (Catalog # A00930-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EML4 at approximately 120KD. The expected band size for EML4 is at 109KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEML4
Western blot analysis of ETS1 using anti-ETS1 antibody (A00931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: NIH3T3 whole Cell lysates, Lane 2: A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETS1 antigen affinity purified polyclonal antibody (Catalog # A00931-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETS1 at approximately 54KD. The expected band size for ETS1 is at 54KD.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetETS1
Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-1).
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPAX8
Western blot analysis of Tal1 using anti-Tal1 antibody (A00944-2).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTAL1
IHC analysis of Igf2r using anti-Igf2r antibody (A00951-1).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetIgf2r
Western blot analysis of IGF2R using anti-IGF2R antibody (A00951).
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIGF2R
IHC analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Cystatin C was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cystatin C Antibody (A00961-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCST3
Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-3).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetCst3
Western blot analysis of IL-22 using anti-IL-22 antibody (A00963-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysate, Lane 2: mouse HEPA1-6 whole cell lysate, Lane 3: rat PC-12 whole cell lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-22 at approximately 22KD. The expected band size for IL-22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIL22