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IHC analysis of PNP using anti-PNP antibody (A00988-1). PNP was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PNP Antibody (A00988-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Nucleoside phosphorylase/PNP Antibody Picoband(r)A00988-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPNP
Flow Cytometry analysis of HEPG2 cells using anti-Human PNP antibody (A00988-Dyl488).
Product group Antibodies
Boster Bio
Anti-Human PNP DyLight(r) 488 conjugated AntibodyA00988-Dyl488
ApplicationsFlow Cytometry
ReactivityHuman
TargetPNP
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human PNP DyLight(r) 550 conjugated AntibodyA00988-Dyl550
ApplicationsFlow Cytometry
ReactivityHuman
TargetPNP
Western blot analysis of CAV3 using anti-CAV3 antibody (A00990-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 antigen affinity purified polyclonal antibody (Catalog # A00990-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV3 at approximately 22KD. The expected band size for CAV3 is at 17KD.
Product group Antibodies
Boster Bio
Anti-Caveolin-3/CAV3 Antibody Picoband(r)A00990-2
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCAV3
Western blot analysis of Syndecan-1/SDC1 using anti-Syndecan-1/SDC1 antibody (A00991-1).
Product group Antibodies
Boster Bio
Anti-Syndecan-1/SDC1 Antibody Picoband(r)A00991-1
ApplicationsWestern Blot
ReactivityHuman
TargetSDC1
Western blot analysis of TBR2/Eomes using anti-TBR2/Eomes antibody (A00992-1).
Product group Antibodies
Boster Bio
Anti-TBR2/Eomes Antibody Picoband(r)A00992-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetEOMES
Western blot analysis of PLAUR using anti-PLAUR antibody (A00993-1).
Product group Antibodies
Boster Bio
Anti-uPA Receptor/PLAUR Antibody Picoband(r)A00993-1
ApplicationsWestern Blot
ReactivityHuman
TargetPLAUR
Western blot analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody (Catalog # A00993-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 37KD. The expected band size for uPA Receptor is at 37KD.
Product group Antibodies
Boster Bio
Anti-uPA Receptor/Plaur Antibody Picoband(r)A00993-2
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetPlaur
Western blot analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate, Lane 2: mouse small intestine tissue lysate, Lane 3: mouse kidney tissue lysate, Lane 4: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody (Catalog # A00993-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 39KD. The expected band size for uPA Receptor is at 37KD.
Product group Antibodies
Boster Bio
Anti-uPA Receptor/PLAUR Antibody Picoband(r)A00993-3
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetPLAUR
Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 63KD. The expected band size for AMPK alpha 1 is at 63KD.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-3
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPRKAA1
Western blot analysis of Myelin Protein Zero using anti-Myelin Protein Zero antibody (A00997-1).
Product group Antibodies
Boster Bio
Anti-Myelin Protein Zero/MPZ Antibody Picoband(r)A00997-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMPZ
Western blot analysis of GNS using anti-GNS antibody (A00999-1).
Product group Antibodies
Boster Bio
Anti-GNS Antibody Picoband(r)A00999-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetGNS
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