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Western blot analysis of IL22 using anti-IL22 antibody (A00963-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 20KD. The expected band size for IL22 is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL22 Antibody Picoband(r)A00963-2
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetIL22
Western blot analysis of IL22 using anti-IL22 antibody (A00963-3).
Product group Antibodies
Boster Bio
Anti-IL22 Antibody Picoband(r)A00963-3
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetIl22
Western blot analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1).
Product group Antibodies
Boster Bio
Anti-BAT3/BAG6 Antibody Picoband(r)A00967-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG6
Western blot analysis of NFAT1 using anti-NFAT1 antibody (A00969). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT1 antigen affinity purified polyclonal antibody (Catalog # A00969) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT1 at approximately 100KD. The expected band size for NFAT1 is at 100KD.
Product group Antibodies
Boster Bio
Anti-NFAT1/NFATC2 Antibody Picoband(r)A00969
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNFATC2
Flow Cytometry analysis of THP-1 cells using anti-HDAC4 antibody (A00971-1). Overlay histogram showing THP-1 cells stained with A00971-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC4 Antibody (A00971-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-HDAC4 Antibody Picoband(r)A00971-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHDAC4
Western blot analysis of Follistatin using anti-Follistatin antibody (A00972-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Follistatin antigen affinity purified polyclonal antibody (Catalog # A00972-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Follistatin at approximately 40KD. The expected band size for Follistatin is at 38KD.
Product group Antibodies
Boster Bio
Anti-Follistatin/FST Antibody Picoband(r)A00972-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFST
Western blot analysis of ABCC2 using anti-ABCC2 antibody (A00974-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCC2 at approximately 190-250KD. The expected band size for ABCC2 is at 174KD.
Product group Antibodies
Boster Bio
Anti-MRP2/ABCC2 Antibody Picoband(r)A00974-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse
TargetABCC2
Western blot analysis of CLPX using anti-CLPX antibody (A00978-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPX antigen affinity purified polyclonal antibody (Catalog # A00978-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPX at approximately 69KD. The expected band size for CLPX is at 69KD.
Product group Antibodies
Boster Bio
Anti-CLPX Antibody Picoband(r)A00978-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetCLPX
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CLPX DyLight(r) 488 conjugated AntibodyA00978-Dyl488
ApplicationsFlow Cytometry
ReactivityHuman
TargetCLPX
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CLPX DyLight(r) 550 conjugated AntibodyA00978-Dyl550
ApplicationsFlow Cytometry
ReactivityHuman
TargetCLPX
Western blot analysis of RanBP2 using anti-RanBP2 antibody (A00981).
Product group Antibodies
Boster Bio
Anti-RanBP2 Antibody Picoband(r)A00981
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRANBP2
Western blot analysis of NEDD4 using anti-NEDD4 antibody (A00984-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody (Catalog # A00984-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4 at approximately 149KD. The expected band size for NEDD4 is at 149KD.
Product group Antibodies
Boster Bio
Anti-NEDD4 Antibody Picoband(r)A00984-2
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNEDD4
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